Structure of Integrator-PP2A bound to a paused RNA polymerase II-DSIF-NELF-nucleosome complex
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8rbx
Q-score: 0.037
Fianu I, Ochmann M, Walshe JL, Dybkov O, Cruz JN, Urlaub H, Cramer P
Nature (2024) [ Pubmed: 38570683 DOI: doi:10.1038/s41586-024-07269-4 ]
- Integrator complex subunit 4 (108 kDa, Protein from Homo sapiens)
- Integrator complex subunit 7 (107 kDa, Protein from Homo sapiens)
- Template dna (56 kDa, DNA from synthetic construct)
- Negative elongation factor c/d (65 kDa, Protein from Homo sapiens)
- Rna polymerase ii subunit k (7 kDa, Protein from Sus scrofa)
- Integrator complex subunit 1 (244 kDa, Protein from Homo sapiens)
- Integrator complex subunit 15 (50 kDa, Protein from Homo sapiens)
- Integrator complex subunit 5 (108 kDa, Protein from Homo sapiens)
- Nelf-b,negative elongation factor b,negative elongation factor b,negative elongation factor b,negative elongation factor b,negative elongation factor b (68 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Integrator complex subunit 6 (100 kDa, Protein from Homo sapiens)
- Non-template dna (45 kDa, DNA from synthetic construct)
- Dsif (Complex from Trichoplusia ni)
- Unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk (1 kDa, Protein from Homo sapiens)
- Integrator complex subunit 13 (80 kDa, Protein from Homo sapiens)
- Integrator complex subunit 14 (57 kDa, Protein from Homo sapiens)
- Transcription elongation factor spt5 (121 kDa, Protein from Homo sapiens)
- Serine/threonine-protein phosphatase 2a catalytic subunit alpha isoform (35 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Sus scrofa)
- Integrator-pp2a complex and nelf (Complex from Homo sapiens)
- Histone h2b type 1-j (13 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase subunit beta (134 kDa, Protein from Sus scrofa)
- Pre-termination complex (1 MDa, Complex)
- Manganese (ii) ion (54 Da, Ligand)
- Serine/threonine-protein phosphatase 2a 65 kda regulatory subunit a alpha isoform (65 kDa, Protein from Homo sapiens)
- Dss1 (3 kDa, Protein from Trichoplusia ni)
- Integrator complex subunit 9 (73 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb11-a (13 kDa, Protein from Sus scrofa)
- Integrator complex subunit 10 (82 kDa, Protein from Homo sapiens)
- Rna polymerase ii subunit d (16 kDa, Protein from Sus scrofa)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Sus scrofa)
- Rna and dna (Complex from Sus scrofa)
- Integrator complex subunit 8 (113 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit e (24 kDa, Protein from Sus scrofa)
- Integrator complex subunit 2 (134 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Integrator complex subunit 11 (67 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase subunit (217 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Sus scrofa)
- Negative elongation factor a (57 kDa, Protein from Homo sapiens)
- Unk-unk-unk-unk-unk-unk-unk-unk (698 Da, Protein from Sus scrofa)
- Dna-directed rna polymerase ii (Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Sus scrofa)
- Rna (5 kDa, RNA from synthetic construct)
- Histone h2a type 1 (14 kDa, Protein from Homo sapiens)
- Nucleosome (Complex from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Sus scrofa)
- Histone h3.3c (15 kDa, Protein from Homo sapiens)
Structure of Integrator-PP2A-SOSS-CTD post-termination complex
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8rbz
Q-score: 0.293
Fianu I, Ochmann M, Walshe JL, Dybkov O, Cruz JN, Urlaub H, Cramer P
Nature (2024) [ Pubmed: 38570683 DOI: doi:10.1038/s41586-024-07269-4 ]
- Integrator complex subunit 4 (108 kDa, Protein from Homo sapiens)
- Integrator complex subunit 7 (107 kDa, Protein from Homo sapiens)
- Dss1 (Complex from Trichoplusia ni)
- Integrator complex subunit 1 (244 kDa, Protein from Homo sapiens)
- Rna polymerase ii c-terminal domain peptides (Complex from Sus scrofa)
- Integrator complex subunit 5 (108 kDa, Protein from Homo sapiens)
- Integrator complex subunit 15 (50 kDa, Protein from Homo sapiens)
- Integrator complex subunit 6 (100 kDa, Protein from Homo sapiens)
- Unk-unk-unk-unk-unk-unk-unk-unk-unk (783 Da, Protein from Sus scrofa)
- Integrator complex subunit 14 (57 kDa, Protein from Homo sapiens)
- Integrator complex subunit 13 (80 kDa, Protein from Homo sapiens)
- Serine/threonine-protein phosphatase 2a catalytic subunit alpha isoform (35 kDa, Protein from Homo sapiens)
- Post-termination complex (1 MDa, Complex)
- Integrator complex subunit 11 (67 kDa, Protein from Homo sapiens)
- Dss1 (3 kDa, Protein from Trichoplusia ni)
- Serine/threonine-protein phosphatase 2a 65 kda regulatory subunit a alpha isoform (65 kDa, Protein from Homo sapiens)
- Soss complex subunit c (11 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase subunit (1 kDa, Protein from Sus scrofa)
- Integrator complex subunit 9 (73 kDa, Protein from Homo sapiens)
- Integrator complex subunit 10 (82 kDa, Protein from Homo sapiens)
- Integrator complex subunit 3 (118 kDa, Protein from Homo sapiens)
- Soss complex subunit b1 (22 kDa, Protein from Homo sapiens)
- Integrator complex subunit 8 (113 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Integrator complex subunit 2 (134 kDa, Protein from Homo sapiens)
- Integrator-pp2a complex and nabp2 and inip (Complex from Homo sapiens)
- Manganese (ii) ion (54 Da, Ligand)
Structure of Integrator-PP2A complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8rc4
Q-score: 0.036
Fianu I, Ochmann M, Walshe JL, Dybkov O, Cruz JN, Urlaub H, Cramer P
Nature (2024) [ Pubmed: 38570683 DOI: doi:10.1038/s41586-024-07269-4 ]
- Integrator complex subunit 4 (108 kDa, Protein from Homo sapiens)
- Integrator complex subunit 7 (107 kDa, Protein from Homo sapiens)
- Dss1 (Complex from Trichoplusia ni)
- Dss1 (3 kDa, Protein from Trichoplusia ni)
- Integrator complex subunit 1 (244 kDa, Protein from Homo sapiens)
- Integrator complex subunit 15 (50 kDa, Protein from Homo sapiens)
- Integrator complex subunit 5 (108 kDa, Protein from Homo sapiens)
- Integrator-pp2a complex (1 MDa, Complex)
- Integrator-pp2a complex (Complex from Homo sapiens)
- Integrator complex subunit 13 (80 kDa, Protein from Homo sapiens)
- Integrator complex subunit 14 (57 kDa, Protein from Homo sapiens)
- Serine/threonine-protein phosphatase 2a catalytic subunit alpha isoform (35 kDa, Protein from Homo sapiens)
- Serine/threonine-protein phosphatase 2a 65 kda regulatory subunit a alpha isoform (65 kDa, Protein from Homo sapiens)
- Integrator complex subunit 9 (73 kDa, Protein from Homo sapiens)
- Integrator complex subunit 10 (82 kDa, Protein from Homo sapiens)
- Integrator complex subunit 8 (113 kDa, Protein from Homo sapiens)
- Integrator complex subunit 6 (101 kDa, Protein from Homo sapiens)
- Integrator complex subunit 2 (134 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Integrator complex subunit 11 (67 kDa, Protein from Homo sapiens)
- Manganese (ii) ion (54 Da, Ligand)
Neurokinin B bound to active human neurokinin 3 receptor in complex with Gq
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8jbg
Q-score: 0.569
Sun W, Yang F, Zhang H, Yuan Q, Ling S, Wang Y, Lv P, Li Z, Luo Y, Liu D, Yin W, Shi P, Xu HE, Tian C
Cell Discov (2023) 9 pp. 66-66 [ DOI: doi:10.1038/s41421-023-00564-w Pubmed: 37391393 ]
- Guanine nucleotide-binding protein gq (g324) (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Rattus norvegicus)
- Scfv16 nanobody (32 kDa, Protein from Mus musculus)
- Neurokinin-b (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Neuromedin-k receptor (nk3r-pfastbac1) (158 kDa, Protein from Homo sapiens)
- Neurokinin b bound to active human neurokinin 3 receptor in complex with gq heterotrimer (Complex from Homo sapiens)
Substance P bound to active human neurokinin 3 receptor in complex with Gq
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8jbh
Q-score: 0.575
Sun W, Yang F, Zhang H, Yuan Q, Ling S, Wang Y, Lv P, Li Z, Luo Y, Liu D, Yin W, Shi P, Xu HE, Tian C
Cell Discov (2023) 9 pp. 66-66 [ DOI: doi:10.1038/s41421-023-00564-w Pubmed: 37391393 ]
- Nk3r-pfastbac1 (158 kDa, Protein from Homo sapiens)
- Substance p bound to active human neurokinin 3 receptor in complex with gq heterotrimer (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Rattus norvegicus)
- Peptide from protachykinin-1 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein gq subunit alpha (g324) (41 kDa, Protein from Homo sapiens)
- Scfv16 nanobody (32 kDa, Protein from Mus musculoides)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
The cryo-EM structure of insect gustatory receptor Gr43a from Drosophila melanogaster
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8jm9
Q-score: 0.593
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 8jma
Q-score: 0.637
The cryo-EM structure of insect gustatory receptor Gr43a I418A from Drosophila melanogaster
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8x82
Q-score: 0.555
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8x83
Q-score: 0.56
Ma D, Hu M, Yang X, Liu Q, Ye F, Cai W, Wang Y, Xu X, Chang S, Wang R, Yang W, Ye S, Su N, Fan M, Xu H, Guo J
Science (2024) 383 pp. eadj2609-eadj2609 [ Pubmed: 38305684 DOI: doi:10.1126/science.adj2609 ]
- Sodium ion (22 Da, Ligand)
- Gustatory receptor gr43a i418a (Cellular component from Drosophila melanogaster)
- Water (18 Da, Ligand)
- Gustatory receptor for sugar taste 43a (49 kDa, Protein from Drosophila melanogaster)
- Beta-d-fructofuranose (180 Da, Ligand)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8x84
Q-score: 0.525
Ma D, Hu M, Yang X, Liu Q, Ye F, Cai W, Wang Y, Xu X, Chang S, Wang R, Yang W, Ye S, Su N, Fan M, Xu H, Guo J
Science (2024) 383 pp. eadj2609-eadj2609 [ Pubmed: 38305684 DOI: doi:10.1126/science.adj2609 ]
- Gustatory receptor gr43a_i418a (Cellular component from Drosophila melanogaster)
- Calcium ion (40 Da, Ligand)
- Water (18 Da, Ligand)
- Gustatory receptor for sugar taste 43a (49 kDa, Protein from Drosophila melanogaster)
- Beta-d-fructofuranose (180 Da, Ligand)